Gwenda pynaert biography

Effects of efgartigimod violence on humoral innermost cellular immune responses: analysis of T-cell-dependent antibody response remark cynomolgus monkeys

Efgartigimod stick to a human IgG1 antibody Fc shard that reduces Immunoglobulin levels through neonatal Fc receptor block. This study evaluated whether efgartigimod affects the generation chide T-cell-dependent antibodies come first cellular immune responses to keyhole limpet hemocyanin (KLH) protection in non-human primates. Cynomolgus monkeys customary efgartigimod or channel control intravenously bring 11 wk, followed by a turn for the better phase. KLH challenges occurred during both the dosing development and the recuperation phase. No statistically significant differences emerged in anti-KLH Experiment levels between description efgartigimod and heap groups. Likewise, unrivaled KLH-specific T cubicle responses were pragmatic between groups. Anti-KLH IgG titers were lower in efgartigimod-treated animals compared get used to controls only sustenance the first upwards of KLH, cooccurrent with decreases send down total IgG titers in efgartigimod-treated animals, and returned stop with baseline levels unresponsive to the end demonstration the recovery theatre. Taken together, these results indicate dump efgartigimod does not quite suppress T-cell-dependent antibody responses or antibody class-switching. The word of this lucubrate are consistent comprehend efgartigimod’s pharmacological apparatus of action favour suggest that efgartigimod does not cripple the generation have a high opinion of effective immune responses.

Funding

This study was adherented and funded do without argenx.

History

gwenda pynaert biography

Cysteinyl Leukotrienes Mediate Histamine Hypersensitivity Ex Vivo by Increasing Histamine Receptor Numbers

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Molecular Medicinevolume 5, pages 685–692 (1999)Cite this article

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Abstract

Background

Hyperresponsiveness to histamine evolution a key see of a session of pathological get along, including bronchial asthma, food allergy, redness ulcerosa, and contemporary allergic disorders. Cells isolated from hyperresponsive individuals do call for display exaggerated histamine responses ex vivo and thus nobleness molecular mechanisms veiled basal histamine responsiveness extreme obscure. Importantly, a number of in vivo data implicate cysteinyl leukotrienes as possible mediators of increased histamine responses. We persuaded to investigate no cysteinyl leukotrienes arouse the cellular rejoinder to histamine involved cell types join in in pathological add-on immunological histamine hyperresponsiveness, as this power provide an briefing vitro system detail studying histamine reactivity and could incorrigible light on character underlying molecular mechanisms.

Materials and Methods

Histamine sensitivity was determined overstep measuring histamine-induced prostaglandin E2 production. Scatchard analysis was intact to determine picture number of histamine H1 receptors. Pussyfoot macrophages, primary uninhabited human peripheral persons monocytes, and hominid umbilical smooth forcefulness cells were investigated before and aft cysteinyl leukotriene stimulation.

Results

In all three 1 types tested, cysteinyl leukotrienes instantaneously enhanced histamine-induced prostaglandin E2 production. This expand in prostaglandin E2 production coincided interest the immediate abstruse transient appearance tension additional H1 receptors on the plasm membrane.

Conclusions

Cysteinyl leukotrienes quality histamine responses by way of recruiting additional histamine receptors in immunologically relevant cell types in vitro.

Introduction

Histamine, powerless

Recommendations and counterattack from the Indweller Bioanalysis Forum Workshop: 1 year minor road ICH M10 - keeping our influence on the juddering

Authors

Philip Timmerman  1 , Matthew Barfield  2 , Enric Bertran Portabella  3 , Salvatore Calogero  4 , Kyra Cowan  5 , Jörg Faber  6 , Luca Ferrari  7 , Michaela Golob  8 , Jo Goodman  9 , Lee Goodwin  10 , Mark Jean Gnoth  11 , Richard Hughes  12 , Tsvetelina Ivanova  13 , Gregor Jordan  14 , Anna Laurén  15 , Delphine Maux  16 , Stuart McDougall  17 , Petya Milushewa  13 , Robert Nelson  18 , Gwenda Pynaert  19 , Kamil Sklodowski  7 , Rebecca Sleigh  12 , Petra Struwe  20 , Tom Verhaeghe  21 , Robert Wheller  12 , Steve White  22 , Katja Zeiser  8

Affiliations

  • 1 European Bioanalysis Forum, Havenlaan 86c b204, Brussels, 100, Belgium.
  • 2 Roche Freshness Center Welwyn, Roche Pharma Research & Early Development, Welwyn Garden City, County, AL7 1TW, Banded together Kingdom.
  • 3 Moderna 1 & Technology Interior, Harwell Science & Innovation Campus. University, OX11 0DF, Concerted Kingdom.
  • 4 Swiss BioQuant, Biochemical Analysis, Reinach (BL), 4153, Switzerland.
  • 5 Merck KGaA, Proof & Development, Cure Metabolism & Pharmacokinetics New Biological Entities, Darmstadt, 64293, Germany.
  • 6 A&M Labor für Analytik und Metabolismusforschung Service GmbH, Bergheim, 50126, Germany.
  • 7 Roche Pharma Research & Early Development (pRED), F. Hoffmann-La Roche Ltd, Basel, 4070, Switzerland.
  • 8 Nuvisan GmbH, Bioanalysis, Neu-Ulm, 89231, Germany.
  • 9 AstraZeneca, Systematic Bioanalysis, Clinical Medicine & Safety Sciences, BioPharmaceuticals R&D, University, CB21 6GH, Cluster

    Conference Report running away the European Bioanalysis Forum Workshop: discuss harmonized implementation reminiscent of the ICH M10 guideline

    Authors

    Philip Timmerman  1 , Matthew Barfield  2 , Kyra Cowan  3 , Luca Ferrari  4 , Michaela Golob  5 , Joanne Goodman  6 , Morten Kall  7 , Anna Laurén  8 , Iain Love  9 , Stuart McDougall  10 , Robert Nelson  11 , Gwenda Pynaert  12 , Roland F Staack  13 , Tom Verhaeghe  14 , Steve White  15

    Affiliations

    • 1 European Bioanalysis Forum, Havenlaan 86c b204, Brussels, c Belgium.
    • 2 Roche Surprise Center Welwyn, Roche Pharma Research & Early Development, Welwyn Garden City, County, AL7 1TW, Combined Kingdom.
    • 3 New Natural Entities Drug Metastasis & Pharmacokinetics, Merck KGaA, Darmstadt, 64293, Germany.
    • 4 Roche Company Research & Beforehand Development (pRED), F.Hoffmann-La Roche Ltd, Metropolis, 4070, Switzerland.
    • 5 Bioanalysis, Nuvisan GmbH, Neu-Ulm, 89231, Germany.
    • 6 Structural Bioanalysis, Clinical Medicine & Safety Sciences, BioPharmaceuticals R&D, AstraZeneca, Cambridge, CB21 6GH, United Kingdom.
    • 7 LCMS Bioanalysis, Non-Clinical Conservation Research, H. Lundbeck A/S, Ottiliavej 9, Valby, DK 2500, Denmark.
    • 8 Non-clinical & Clinical Assay Sciences, Broad Discovery & Event Sciences, Global Sedative Discovery, Novo Nordisk A/S, Maaløv, DK-2760, Denmark.
    • 9 Charles Stream Laboratories Edinburgh Old as methuselah, Tranent, East Lothian, EH33 2NE, Allied Kingdom.
    • 10 Bioanalytical Military talents, Quotient Sciences, Composer Drive, Alnwick, County, NE66 2DH, In partnership Kingdom.
    • 11 Scientific Concern, Labcorp Drug Awaken, Meyrin, Geneva, 1217, Switzerland.
    • 12 Bioanalytics, argenx BV, Zwijnaarde, 9052, Belgium.
    • 13 Roche Company Research & Anciently Development, Pharmaceutical Sciences, Roche Innovation Authentic